请尝试以下更新的代码.
public void CreateExcel() { Microsoft.Office.Interop.Excel.Application xlApp = new Microsoft.Office.Interop.Excel.Application(); if (xlApp == null) { MessageBox.Show("Excel is not properly installed!!"); return; } Microsoft.Office.Interop.Excel.Workbook xlWorkBook; Microsoft.Office.Interop.Excel.Worksheet xlWorkSheet; object misValue = System.Reflection.Missing.Value; xlWorkBook = xlApp.Workbooks.Add(misValue); xlWorkSheet = (Microsoft.Office.Interop.Excel.Worksheet)xlWorkBook.Worksheets.get_Item(1); xlWorkSheet.Cells[1, 1] = "ID"; xlWorkSheet.Cells[1, 2] = "Name"; xlWorkSheet.Cells[2, 1] = "1"; xlWorkSheet.Cells[2, 2] = "One"; xlWorkSheet.Cells[3, 1] = "2"; xlWorkSheet.Cells[3, 2] = "Two"; //Here saving the file in xlsx xlWorkBook.SaveAs("d:\\vdfgdfg.xlsx", Microsoft.Office.Interop.Excel.XlFileFormat.xlOpenXMLWorkbook, misValue, misValue, misValue, misValue, Microsoft.Office.Interop.Excel.XlSaveAsAccessMode.xlExclusive, misValue, misValue, misValue, misValue, misValue); xlWorkBook.Close(true, misValue, misValue); xlApp.Quit(); Marshal.ReleaseComObject(xlWorkSheet); Marshal.ReleaseComObject(xlWorkBook); Marshal.ReleaseComObject(xlApp); MessageBox.Show("Excel file created , you can find the file d:\\csharp-Excel.xlsx"); }